The Strategic Plan for Biodiversity (2011–2020), formally adopted by COP in 2010 in Aichi, Japan provided an overarching global framework on biodiversity whose vision is to value, restore, and conserve biodiversity for the benefit of all people by 2050. This retrospective analysis of progress against the 20 Aichi Targets based on the Sixth National Reports and the National Biodiversity Strategy and Action Plans (NBSAPs) provides a comprehensive overview of the efforts made by HKH regional member countries in implementing the Strategic Plan for Biodiversity. The study is also expected to contribute to the CBD process when the parties review progress during CBD COP15. It will also strengthen the mechanisms that can support implementation and monitoring of targets in the post-2020 Global Biodiversity Framework (GBF).