The policy guidelines have been developed to support behavioural change in individuals, households and institutions to strengthen waste segregation at source (WSS). The objective is to facilitate, strengthen, widen and deepen, the adoption of WSS practices among different stakeholders for a sustainable and lasting impact. The recommendations are anchored in a holistic perspective on human behaviour that contextualize WSS as being a behavioural practice and act that is enmeshed with the psychostructural features of the environment. The recommendations have relevance for policies in the broad areas of waste management, circularity, sanitation, urban planning, and product (including food) packaging, amongst others.