Aconitum violaceum Jacq. ex Stapf. is endemic to the Northern Himalayan regions of India, Pakistan and Nepal. To adapt and endure the rigorous stressful situations, phenotypic features of a specific plant species vary across different climatic conditions and elevations. The aim of this study was to reveal the impact of altitude and environmental conditions on the morphological attributes of a threatened species A. violaceum. Plants at high elevations (Maan-man, 4010 m asl) were short; whereas the plants grown at ecological conditions of population-I (Khawous, 3220 m asl) showed better growth and development in all morphological attributes. It has been noticed that there is a considerable reduction in the plant height, rhizome, leaf dimension, flower number and leaf number at higher eleva-tions. Regression analysis and Pearson's correlation coefficient demonstrat-ed a positive relationship between plant height and all other morphological attributes. Principal component analysis (PCA) revealed that the habitat, ecological conditions and elevations of population-I (Khawous) and Popula-tion-II (Numsuru) were proved to be relatively better for the luxuriant growth and development of an endemic plant species A. violaceum. From the observations, it can be inferred that A. violaceum grows successfully at an elevation ranges between 3000 to 3500 m asl. This study provides a com-prehensive account of phenotypic variability concerning environmental conditions and suggested a suitable environment for sustainable cultivation of this important medicinal plant species in the Ladakh Himalaya. © 2022 Horizon e-Publishing Group. All rights reserved.