The Far-Eastern Himalayan Landscape, a global biodiversity hotspot spread across parts of India, Myanmar, and China, holds great potential for the long-term conservation of tiger populations. National-level efforts aimed at tiger conservation, such as the creation of protected areas, have been critical for the survival of key tiger populations. However, for tigers to continue to survive into the future, it is important that these key populations remain connected with each other, particularly in this transboundary landscape. This requires greater regional and cross-border co-operation in conservation research, monitoring, and planning to protect habitats and corridors. Here, we review and synthesize the available literature on two decades of conservation efforts aimed at the study and conservation of tigers, their co-predators, and prey in the landscape to understand research trends, identify knowledge gaps, and suggest priority areas for future research and conservation interventions. This review could be useful for formulating conservation plans and actions that could help in the recovery of tiger populations; for identifying, restoring, and securing connectivity between key tiger habitats; and for addressing other key threats, such as habitat loss, poaching, and trade in wildlife parts.