This manual provides the materials needed to deliver a basic training in access and benefit sharing (ABS) of genetic resources and associated traditional knowledge as provided for under the Convention on Biological Diversity. The background, key components and procedures, and obligations, rights, and responsibilities of the different actors involved are presented in twenty sessions, supplemented by extensive resource materials and sources for further study. The manual is intended to be used for training trainers, who can then multiply the learning across the region, but can also be used to provide basic knowledge to graduate students of related disciplines. We hope that it will help raise awareness about the ABS regime among the policy makers, development workers, community organisations, and individuals involved in the ABS process.