A region-specific species checklist is an important resource for biodiversity documentation and conservation. This review provides an updated mammal species checklist for the biodiversity hotspots of the Darjeeling-Sikkim landscape in Eastern Himalaya. The list was compiled by systematically reviewing 94 available publications spanning 178 years from 1841 to 2019, for mammals from the region. The species checklist is envisioned to aid in understanding the current status of mammal records, historical distribution, ranked conservation status of mammals, and research gaps. A total of 173 mammal species under 11 orders and 33 families, including the recently upgraded taxon, Sikkim Pika Ochotona sikimaria was enlisted. There are 25 species included in the IUCN threatened categories, 58 species listed in the CITES Appendices, and 112 species included in the schedules of the Wildlife (Protection) Act, 1972 in India. Although mammals receive the maximum research attention in the landscape, small mammals and bats have rarely been subjected to systematic studies in recent years. © Naulak & Pradhan 2020.