Two new species of the genus Trilacuna Tong & Li, 2007, T. jinyun Tong, Zhang & Li, sp. nov. (♂♀) and T. jiuchi Tong, Zhang & Li sp. nov. (♂♀), are described from Chongqing, China. Photos of the habitus and copulatory organs are provided. © 2021, Museum National d'Histoire Naturelle. All rights reserved.