Remote sensing estimations of glacier flow velocity could provide effective methods for the long-term monitoring of glacier flow velocity. This paper calculated the velocity in the line-of-sight (LOS) direction by combining DInSAR and offset-tracking technology with ascending and descending Sentinel-1 images of the Urumqi Glacier No.1 from 2016 to 2017. Meanwhile, the velocity in the azimuthal direction was obtained by combining MAI and offset-tracking technology. Then, the eastward, northward, and upward flow velocities were retrieved using the Helmert variance component estimation method. Finally, the standard error of the mean and mean errors of surface velocity in non-glaciated areas of the Urumqi Glacier No.1 were calculated to evaluate the accuracy of the results generated by the proposed method. The results showed: (1) The ascending LOS velocity and the descending LOS velocity were 1.812 m/a and −1.558 m/a from 2016 to 2017. The ascending azimuthal and descending azimuthal velocities were 0.978 m/a and −2.542 m/a, respectively. (2) The glacier flow velocities were 2.571 m/a and 1.801 m/a, respectively, for the eastward and northward directions. In the vertical direction, the velocity was −0.554 m/a. (3) The accuracy of the results generated by the proposed method were 0.028 m/a, 0.085 m/a, and 0.063 m/a in the east, north, and vertical directions. Therefore, it is suitable to use ascending and descending Sentinel-1 images and the study method proposed in this paper to estimate the surface flow velocity of mountain glaciers. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.