While carrying out identification of Barleria L. (family Acanthaceae) collected from various places of Nepal and preserved in National Herbarium and Plant Laboratories, Department of Plant Resources, Godawari, Lalitpur, Nepal (KATH), we came across some specimens belonging to one interesting species. During the identification, this species did not match with any species of Barleria already reported from Nepal (Hara and Shakya, 1982; Press et al., 2000; Bista et al., 2001). It has been identified as Barleria prionites L. which is new addition to the flora of Nepal. This species was collected from Nepalganj, Banke district, West Nepal at 181m.